Composite
Part:BBa_K3145003
Designed by: Yazmin Camacho Group: iGEM19_US_AFRL_CarrollHS (2019-09-28)
J18912-bFMO
This plasmid contains bacterial Flavin-containing monooxygenase (bFMO) BBa_K2598027 with a T7 promoter + RBS + Start codon upstream BBa_J18912 of it. It will constitutively express the bFMO gene to produce a visible indigo color.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 240
Illegal AgeI site found at 263
Illegal AgeI site found at 567
Illegal AgeI site found at 897 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 20
[edit]
Categories
Parameters
None |