Composite

Part:BBa_K3145003

Designed by: Yazmin Camacho   Group: iGEM19_US_AFRL_CarrollHS   (2019-09-28)


J18912-bFMO

This plasmid contains bacterial Flavin-containing monooxygenase (bFMO) BBa_K2598027 with a T7 promoter + RBS + Start codon upstream BBa_J18912 of it. It will constitutively express the bFMO gene to produce a visible indigo color.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 240
    Illegal AgeI site found at 263
    Illegal AgeI site found at 567
    Illegal AgeI site found at 897
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 20


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